VDJ library construction was performed according to the manufacter’s instructions (single cell 5’ v1 protocol, 10x Genomics). Antibodies used for sorting: CD45 (I3/3.2), CD3 (17A2) The populations were then recombined in a 2:1:1 ratio, and 10,000 cells from each condition (TTx-treated and control) were loaded onto the 10X Chromium System for sequencing. Samples were then FACS-sorted into three populations: CD45+CD3+ (T cells), CD45+CD3- (non-T immune cells), CD45-CD3- (tumor and stroma). Tumors were then filtered through 70 µM filters into a single-cell suspension. Mice were treated with 50 µg anti-CD40 (FGK4.5, BioXCell), 200 µg anti-CSF1R (m CSF1R.2 m.G1- D265A, Bristol Myers Squibb) and 200 µg anti-PD-1 (RMP1-14, BioXCell) 7 days post-tumor initation.Ģ4 hours post-treatment (if applicable), untreated and TTx-treated tumors for scRNA-seq were minced in RPMI with 2% FBS, then incubated with agitation at 37C, with 1 mg/mL Collagenase IV (C5138, Sigma-Aldrich) and 0.1 mg/mL DNAse I (10104159001, Roche) for 30 minutes. YUMMER1.7 (YR) tumor (8 days post-tumor initiation, untreated), scTCR-seqįor subcutaneous tumors, C57BL6/J mice were injected with 0.5x10^6 YUMMER1.7 cells (BrafV600E/ Pten-/-Cdkn2a -/- UV irradiated melanoma cell line). What is new in VirtualDJ 7.GEO help: Mouse over screen elements for information. * GenuisDJ: automatically fill your folders with the latest hits depending on what you usually play * MusicGroup: get live recommendations based on what you play and the feedback from millions of other DJs around the world * Netsearch: search and play any song from an online database of more than 8 million tracks * Optional automatic playlist mixing: VirtualDJ recognizes the style of the music (techno, hip hop, lounge) and adapts the mix accordingly * Compatibility and customizable mapping with most external MIDI and HID controllers (more than 80 included, many more downloadable) * VDJScript: powerful macro language used in skins, shortcuts, or controller mapping * Fully customizable skin engine and shortcut macro engine * Multi-channel sound card, dual-sound card or Y-splitter output for real-time monitoring or external mixer use * Ready-to-burn recording of a DJs mix to CD * Automatic Hot-Swap of external hard drives * Compatible with iTunes playlists (iTunes DRM files not supported) * Song database engine with easy-to-use search feature * Infinite number of video effects applied simultaneously * Video mix with windowed or FULL SCREEN TV output using 2nd video output * VST effects compatibility (PC version Only) * Beat-aware effect plugins (included: beatgrid, flippin doubles, vocal remover, frequency filter, flanger, backspin, brake, etc.) * Infinite number of cue points per song saved for instant recall each time the song is loaded * Automatic first beat and last beat detection * Time-Stretch and Master Tempo Pitch algorithms * Synchronized sampler with 12 instant record and playback slots * Automatic seamless and beat-aware LOOP functionality * BeatLock engine for keeping songs in-sync and in-time * One-click beat matching and synchronization (FAME algorithm) 3 band equalizer High, Mid, and Low with Kill and +/-30db gain Pitch control with Master Tempo (from -100% to +100%) Standard controls (play, pause, stop, cue) * Up to 99 independent zero-latency players with: Lastly, enter the new era of DJs mixing video enhanced songs (DVD, DivX, MPEG.) which can be sent to monitors, TVs, a projector for display on a giant screen. The vinyl controls will let you scratch like on a real turntable, except that with the beatlock engine your scratches will never end out of the beat.Īdd to that the infinite number of cue points a DJ can save for each song and large collection of wonderful automatically beat-synchronized effects.Īnd with VirtualDJ's large collection of skin interfaces to suit everybody from the beginner to the professional DJ, the possibility to record the DJ's mix to then burn to CDs, to broadcasting on the Internet and/or the DJ's own radio station, to use headphones to preview the song, or use an external mixer to perform in a club VirtualDJ is a DJ's ULTIMATE mix software. The visual representation and the cues allow a DJ to clearly see the song structure, and never be surprised by a break. The automatic seamless loop engine and synchronized sampler lets the DJ perform astounding remixes live, with no preparation at all. With VirtualDJ's breakthrough BeatLock engine, songs will always stay in beat, and the DJ works their mixes incredibly faster than they ever could. VirtualDJ is the hottest AUDIO and VIDEO mixing software, targeting DJs from the bedroom, mobile, and professional superstars like Carl Cox.
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